TBX18 and infection: Additionally, NMVMs treated with a higher dose of each TBX18 vector (3 ng per 1,000 cells mRNA, multiplicity of infection (MOI) 5 Adv) showed a significantly higher number of GFP+ myocytes compared with respective controls, yet were similar to NMVMs treated with lower doses of mRNA TBX18 or Adv TBX18 (Extended Data Fig. 2f,g).