The PLK4 inhibitor centrinone and the shRNA knockdown induced AML cell apoptosis by increasing the activation of Caspase-3/poly ADP-ribose polymerase (PARP), and caused the G2/M phase cell cycle arrest by decreasing the expression of cell cycle-related proteins such as Cyclin A2, Cyclin B1, and Cyclin-dependent kinase 1 (CDK1)30. This evidence concerns the gene PLK4 and acute myeloid leukemia.