DDX3X and colorectal cancer: We depleted endogenous DDX3X using an inducible degron system, employing a male-derived colorectal cancer HCT 116 cell line with stably integrated auxin-activated OSTIR1 machinery, where the endogenous DDX3X gene was tagged with a degradation tag (Natsume et al. 2016; Calviello et al. 2021).