This applicability of OnCELISA was then used to detect IL-6-expressing cells using flow cytometry in atherosclerotic plaque-containing mouse aortae digested into single-cell suspensions.109 This demonstrates a clear application in atherosclerosis in which the OnCELISA technology could be used to detect key atherosclerotic inflammatory proteins specifically released from macrophages for the improved diagnosis of atherosclerosis. This evidence concerns the gene PROS1 and atherosclerosis.