As a result, the most significant KEGG pathways were identified, including Leishmaniasis, Chemokine signaling pathway, Complement and coagulation cascades, NF-kappa B signaling pathway, TNF signaling pathway, RIG-I-like receptor signaling pathway, Viral protein interaction with cytokine and cytokine receptor, Th17 cell differentiation, Th1 and Th2 cell differentiation, Growth hormone synthesis, secretion and action, NOD-like receptor signaling pathway and JAK-STAT signaling pathway (Figure 2F). This evidence concerns the gene TNF and leishmaniasis.