CD28 and ovarian carcinoma: To demonstrate the mechanistic effect of CAR-NK cells in ovarian cancer, the authors have generated MSLN- or CD19-CAR molecule by cloning the genes of anti-MSLN scFv, and anti-CD19 scFv, along with CD8 leader, CD8 hinge, CD28 transmembrane, and composite CD28-CD137-CD3ζ intracellular signaling domains into the lentiviral vector pWPXLd-2A-eGFP through PmeI and SpeI cloning sites.