To further explore the potential role of elevated ZBP1 and NLRP6 expression in oHSV infection in tumor cells, we generated ZBP1-deficient 4T1 and 4MOSC1 cells, as well as NLRP6-deficient 4T1 and 4MOSC1 cells, using CRISPR/Cas9-based gene editing. This evidence concerns the gene NLRP6 and neoplasm.