Glis2-ASO treated Pkd1fl/fl; UBCCre-ERT2 mice showed similar presence of sporadic SA-β-gal cell staining that was qualitatively reduced compared to untreated cystic kidneys indicating that Glis2-ASO did not result in increased lysosomal activity as detected by SA-β-gal staining (Supplementary Fig. 24d). Here, GLIS2 is linked to Renal cyst.