Together, here, we validated the efficacy of TGFβ to induce fibrotic and inflammation genetic programs and highlighted that SETDB1 silencing alters the transcription of many secreted factors (secretome) and ECM components, attenuating the deleterious effect of TGFβ overactivation in DMD myotubes. Here, TGFB1 is linked to Duchenne muscular dystrophy.