The infiltrate of CD3 positive cells was higher but not statistically significant in high grade penile squamous cell carcinomas (p = 0.08) (Fig 2A), while the number of cells expressing FOXP3, CD68 and CD163 were significantly higher in the high-grade tumors when compared with the low-grade tumors, Fig 2C–2E respectively. This evidence concerns the gene CD163 and squamous cell carcinoma.