MDBK cells incubated with BRSV at 4°C for 1 h were transferred to 37°C for 30 min, the localization of EEA1 and BRSV G protein was analyzed by IFA and confocal microscopy, the result showed clear co-localization of EEA1 and BRSV G protein after 30 min of infection (Figure 1I). The gene discussed is EEA1; the disease is infection.