ALS-increased DEGs with increased expression in endstage G93A motor neurons from both strains included C3AR1, RGS1 and NCKAP1L (Supplementary Figure S25A) and such genes were associated with interspecies interaction, blood vessel development and inflammatory response (Supplementary Figure S25C). The gene discussed is NCKAP1L; the disease is amyotrophic lateral sclerosis.