It was possible to identify ALS-increased DEGs upregulated during motor neuron differentiation (e.g., MLC1, ANGPT1, CP; see Supplementary Figures S23A, B, D) as well as ALS-decreased DEGs downregulated during motor neuron differentiation (e.g., EFNA5, LGI2, PAQR9; Supplementary Figures S23C, E). This evidence concerns the gene LGI2 and amyotrophic lateral sclerosis.