Since MNX1 acts as a transcriptional repressor (William et al., 2003; Lee et al., 2008), loss of MNX1 activity in ALS motor neurons could have a transcriptional derepression effect, a common feature of genetic disease (Gabellini et al., 2003), which may account for increased expression of some DEGs in this study. Here, MNX1 is linked to amyotrophic lateral sclerosis.