The animals were sacrificed, and the tumor tissue from stressed animals exhibited increased mRNA expression of the EMT-related genes Twist1, Twist2, Zeb1, Zeb2, Slug, and PLAGL2 (Fig. 1i) and increased protein expression of PLAGL2, Ki67 (a proliferation marker), N-cadherin, and Vimentin (Fig. 1j–m, and Supplementary Fig. 1h, i). Here, TWIST2 is linked to neoplasm.