To assess the effects of phosphorylations observed in cancer cells on hLon functions, we mutated the human LONP1 gene to encode phosphorylated or phosphorylation-mimicking variants of human mitochondrial Lon protease lacking the N-terminal MTS and fused to a hexahistidine tag (6 × His) using E. coli expression systems (Supplementary Table S2). The gene discussed is LONP1; the disease is cancer.