Interestingly, there were two CpGs with decreased 5hmC levels and increased gene expression in astrocytoma, ependymoma, and glioneuronal/neuronal tumors: cg18280362 located in the promoter region of CWH43 and cg08278401 located in the promoter region of LRRC72. In addition, we investigated the association between changes in 5mC methylation and gene expression in the embryonal tumors where there were 24 dmCpGs associated with significant changes in gene expression (Supplementary Fig. 16). The gene discussed is LRRC72; the disease is ependymoma.