For in vivo studies, we utilized immunocompetent C3H/HeOuJ mice for subcutaneous tumor growth by injecting murine H&N cancer cell lines (SCCVII and MEER) and determined anti‐tumor activities of several combinations of immune checkpoint inhibitors, including anti‐CTLA‐4 antibody and APG‐157. This evidence concerns the gene CTLA4 and neoplasm.