In the bilateral tumor model, we excised one flank tumor before (day 0), or 2, 4 or 6 days after anti-CTLA-4 and anti-PD-L1 treatment for bulk TCRβ sequencing in two mouse models, AB1 mesothelioma and RENCA renal cancer (Figure 3(a)). The gene discussed is CTLA4; the disease is renal carcinoma.