On the one hand, the TLR3 signal sequentially activated IRF3 and NF‐κB after poly I:C (a TLR3 agonist) stimulation, which resulted in the release of IL‐6 and C‐C motif chemokine ligand 5(CCL5), as well as the enhancement of cancer cell migration. This evidence concerns the gene NFKB1 and cancer.