The same study also revealed that the knockdown of ANO1 significantly decreased p‐p38 and phosphorylation extracellular signal‐regulated kinases 1 and 2 (p‐ERK1/2) and that the cell cycle protein D1 was reduced, but p‐JNK was not altered, which suggests that in HCC cells, the knockdown of ANO1 can regulate the cell cycle and inhibit cell proliferation and metastasis through the Mitogen‐activated protein kinase (MAPK) signalling pathway. This evidence concerns the gene MAPK3 and hepatocellular carcinoma.