In the present study, we demonstrate that engineered T cells expressing scFv‐huIL‐13Rα2 CAR proliferate, bind target antigen, and exhibit the cytotoxic activity to IL‐13Rα2 positive tumour cells, but not to IL‐13Rα2 negative tumour cells or IL‐13Rα2 gene silenced tumour cells. This evidence concerns the gene IL13RA2 and neoplasm.