Using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays, cytotoxicity of DE and DAES with or without SO1861 (125 ng/mL) was determined on the EGFR-positive colon cancer cell line HCT 116 and the mammary gland carcinoma cell line MDA-MB-453, which expresses only low amounts of EGFR [25, 26] (see also Additional file 6). The gene discussed is EGFR; the disease is malignant colon neoplasm.