On the other hand, an analysis of changes in immune checkpoint receptors over time showed significant decreases in the expression of immune checkpoint receptors programmed death receptor-1 (PD-1) and CD112R in hypothalamic microglia at the cachexia stage, but not at the pre-cachexia stage (Fig. 3E, F, K-L, Unpaired t-test, *p < 0.05, ***p < 0.001 vs. control group). The gene discussed is PVRIG; the disease is Cachexia.