The results showed that the percentages of CD8+ T and CD4+ T cells to CD45.2+ cells were significantly decreased at the the pre-cachexia and cachexia stage after transplantation of pancreatic cancer cells (Fig. 4B, D, E, one-way ANOVA with the post-hoc Bonferroni test, $$p < 0.01, $$$p < 0.001 vs. control (pre-cachexia) group, ***p < 0.001 vs. control (cachexia) group). This evidence concerns the gene CD4 and pancreatic neoplasm.