To this end, polymeric nanoparticles with surface conjugation to anti-CD8 antibody were loaded with mRNAs encoding CAR and TCR, which could selectively bind to CD8+ T cells in the circulating blood of murine models bearing human leukemia, prostate cancer, and hepatocellular carcinoma, to initiate receptor-mediated endocytosis. The gene discussed is CD8A; the disease is prostate cancer.