To investigate the impact of the RIG-I signaling pathway on cytokine release and MHC-I upregulation in isiBCL-2-induced cancer cells, U251 cells were pretreated with siRNA-RIG-I, and the mRNA levels of IFN-β, CXCL10, and MHC-I were assessed following isiBCL-2 transfection. The gene discussed is RIGI; the disease is cancer.