We additionally identified nine TFs including CEBPA, FOXA1, FOXA3, FOXK1, FOXN3, FOXP3, NFATC2, PROP1, and SRY, which were classified in a subgroup containing HAL by the cluster analysis, suggesting that these TFs are candidate transcriptional activator(s) regulating HAL. Among the nine TFs, we focused on factors whose expression was correlated with HAL in liver cancer tissues using the TCGA data (cBioportal, https://www.cbioportal.org/), because the negative regulation of HAL by Wnt signaling was most explicit in liver cancer, but not in colorectal cancer7. This evidence concerns the gene PROP1 and liver cancer.