Finally, in the attempt to better understand the mechanism of action of ASNS upregulation in ADPKD, we found that its inhibition blunted the carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase (CAD)-dependent de novo pyrimidine synthesis pathway. This evidence concerns the gene ASNS and autosomal dominant polycystic kidney disease.