Given the major effect of Asns targeting on pyrimidine biosynthesis, in line with prior literature supporting a role for this pathway downstream of ASNS in sustaining proliferation, and given the evidence that silencing of Asns impairs the proliferation of Pkd1−/− cells (Podrini et al, 2018), we tested whether Asns-ASO treatment could hamper cyst expansion in the Tam-Cre;Pkd1ΔC/flox kidneys acting on the proliferative potential of epithelial mutant cells. This evidence concerns the gene STIM1 and cyst.