Methylation levels of SPTBN2 were found to differ significantly between KIRP, PRAD, LIHC, KIRC, Lung squamous cell carcinoma (LUSC), PAAD, HNSC, BLCA, Esophageal carcinoma (ESCA), BC, THCA, LUAD, COAD, and UCEC and their corresponding normal tissues (Fig. 4a, p < 0.05). This evidence concerns the gene SPTBN2 and bladder transitional cell carcinoma.