PRMT5 and systemic sclerosis: Further multiplexed immunofluorescence staining was performed by labelling with antibodies against CD45, CD3, CD68 and CD20, which have been reported as markers for pronounced infiltrating immune cell types in SSc.1 3 We found a remarkable increase in immune cell infiltration in the skin of PRMT5 immunised mice, including T cells, macrophages and B cells (online supplemental figure S10A,B).