CD8A and infection: Considering that only early depletion of Treg cells resulted in the increase of parasite-specific effector CD8+ T cells and improved control of parasite replication at day 20 pi, and taking into account that the TSKB20-specific CD8+ T cell response starts to arise around day 10 pi in our infection model (Fig 2D) [28], we hypothesized that Treg cells may play a role at early events of T cell priming during T. cruzi infection.