To further confirm YY2 transcriptional regulation on BUB3, we used mutant YY2 overexpression vectors with a zinc finger mutation constructed previously based on cancer genomics data set from the cBioportal database (Figure 3M).[29, 38] Our results showed that these mutant YY2 failed to regulate BUB3 promoter transcriptional activity (Figure 3N). This evidence concerns the gene YY2 and cancer.