To focus on the donor cell-derived MHCI-peptide complexes as the sole antigen, we generated the H-2Kk mouse T lymphoma cell line BW5147 cells stably expressing single-chain trimers (SCT) engineered with OVA257-264 peptide, β2m, and Kb (SCT/BW5147 cells), with the antigen anchored to the plasma membrane (Figures 5A and 5B). Here, B2M is linked to lymphoma.