To explore the impact of APC-driven actin nucleation on colorectal tumor cell invasion, we have engineered LS174T colorectal (adeno)carcinoma-derived epithelial cell lines stably expressing full-length wild-type APC (APC-WT) or a mutant version impaired in actin nucleation (APC-m4; Figure 1A29), on top of the endogenous copy of full-length APC. The gene discussed is APC; the disease is colorectal neoplasm.