Furthermore, upon detecting cg04552852 (TSPAN4), the 9-bp spaced cg12464638 (TSPAN4) is simultaneously identified, both of which are located within the TSS region of TSPAN4. Both of these positions showed significantly lower methylation in the TB group, with average rates of 34.52% ± 7.65% and 37.37% ± 6.74% for TB, and 41.57% ± 5.33% and 42.94% ± 5.90% for HC, respectively (Figure 6B). The gene discussed is TSPAN4; the disease is tuberculosis.