TSPAN4 and tuberculosis: Lastly, qMSP detected methylation of cg04552852 and cg12464638 from TSPAN4 in 99 whole blood samples, with an AUC of 0.794, specificity of 0.720, and sensitivity of 0.816, illustrating the efficacy of assessing the methylation status of cg04552852 and cg12464638 in whole blood for tuberculosis diagnosis.