Mutations were commonly found in genes known to directly control RBL2/pRb activity during G1/S phase transition, such as Cyclin D1, E2Fs, and CDK4 (average CNA frequency for G1/S regulators in Cluster 2 = 0.601; Supplementary Fig. 9j), all of which are known to be associated with aggressive breast tumours and among the most frequent driver genes18,81. This evidence concerns the gene RB1 and breast neoplasm.