To confirm the involvement of astrocyte Kir4.1 deficit in the impairment of recognition memory in FXS, we induced the conditional deletion of Kir4.1 specifically in hippocampal astrocytes by viral delivery of Cre-GFP under the GFAP promoter into the hippocampus of 3 month-old Kir4.1fl/fl male mice, and tested these mice for NOR. Here, KCNJ10 is linked to fragile X syndrome.