Accordingly, HStCs were exposed to CM from tumour educated bone marrow-derived macrophages (BMDMs), isolated from WT and Grn−/− mice, cancer cell CM, or both, for 4 days prior to interrogation for myMAF (Cthrc1, Spp1, Acta2, Col1a1, Col1a2, Postn) and vMAF (Socs3, Hgf, Pdgfra, Itga8) gene signatures (Fig. 4H). This evidence concerns the gene SPP1 and cancer.