The expression of LRP1, SNRNP25, and the LRP1–SNRNP25 fusion protein was increased compared with that in the empty vector group, proving that the osteosarcoma cell lines SAOS2 and 143B expressing LRP1, SNRNP25, and LRP1–SNRNP25 were successfully generated and could be used for subsequent experiments in vitro (Fig. 2A). The gene discussed is SNRNP25; the disease is osteosarcoma.