Clusters 0, 1, 2, and 4, representing periportal and pericentral hepatocytes, showed similar patterns of changes in canonical pathways (Figs. 6 and 7), which include activation of oncogenic signaling pathways (such as EGF, IGF-1, PDGF, and ILK), activation of macrophages (indicated by nitric oxide and reactive oxygen species production) and hepatic fibrosis, indicating that AEG-1-C75S mutation facilitates oncogenic functions of AEG-1. This evidence concerns the gene EGF and Hepatic fibrosis.