In the validation assay of epitope immunogenicity, we stimulated splenocytes using different peptide pools in vitro with the aim of carrying out a dominant epitope analysis, and the results showed that the E5 epitope peptide combination appeared to be the main reason for the cells being induced to secrete antigen-specific IFN-γ, which suggested that the E5 antigenic epitope may play a role in anti-tumor immunity. This evidence concerns the gene IFNG and neoplasm.