In this study, we examined the impact of piperine, glibenclamide, and scopolamine on the growth of cells and the formation of colonies in the Caki-2 cell line and found that piperine and glibenclamide can effectively agonize the activity of TKTL1, indicating that small molecule drugs of kinase agonists may play a role in tumor treatment, achieving drug repositioning and providing novel ideas for kidney cancer treatment. This evidence concerns the gene TKTL1 and kidney cancer.