The qRT-PCR results showed that the NCL mRNA levels were significantly higher in the GBM cells (U87 and U251) compared to the non-target NHA cells (3.41 ± 1.09 and 2.90 ± 0.03 vs. 1 ± 0.09, p < 0.05, Figure 2B). The gene discussed is NUCLEOLIN; the disease is glioblastoma.