DYRK1A and colonic neoplasm: To estimate the potential off-target effect of the RO5454948 inhibitor against Dyrk1A, the closest relative member of Dyrk1B, two colon cancer cell lines were used; SW620 cells, which express both Dyrk1B and Dyrk1A, and HCT116 cells, which express only Dyrk1A, as controls for off-target effects of the RO5454948 inhibitor.