Among them, the 3-(4-hydroxyphenyl), 5-(3,4-dihydroxyphenyl)-1H-pyrazolo [3,4-b] pyridine inhibitor (compound 8h), which exhibits the highest inhibition for Dyrk1B with IC50 of 3 nM, showed remarkable cytotoxicity in the colon cancer cell lines RKO, HCT116, DLD-1, SW480, and SW620. Here, DYRK1B is linked to colonic neoplasm.