Therefore, to investigate whether the administration of CLA can ameliorate obesity-induced IR through regulating metabolic and immunomodulatory signaling, as well as to elucidate whether the modulation was derived from the function of CLA on gut microbiome, we analyzed the changes in glucose tolerance and homeostasis, insulin sensitivity, lipid profile, inflammatory responses, colonic microbial community and metabolites in C57BL/6J mice induced with IR by being fed a HF diet, comparing those administered with CLA to those without. This evidence concerns the gene INS and hydrops fetalis.