Results from the levels of fasting glucose and FINS, HOMA-IR, GTT and ITT demonstrated that the HF feeding led to an obvious hyperglycemia as well as an impaired glucose tolerance and insulin sensitivity, whereas the supplementation of CLA significantly reduced the fasting glucose and insulin levels, increased glucose tolerance and insulin sensitivity, thereby relieving the IR status in HF mice (Figure 1B–F, p < 0.05). This evidence concerns the gene INS and Impaired glucose tolerance.