The final Stx1 subtype, stx1e, was discovered in 2014 by Probert et al. [21] after Shiga toxin was detected in an Enterobacter cloacae strain isolated from an HUS patient from whom the supernatant of a culture of E. cloacae strain was found to be cytotoxic to Vero cells. The gene discussed is STX1A; the disease is hemolytic-uremic syndrome.