The anti-obesity targets of prosapogenin D were sent to the DAVID tool, which showed the ranking top ten KEGG pathways of prosapogenin D; the KEGG signaling pathway enrichment results demonstrated that similar to the results of all active saponins in PG, anti-obesity targets of prosapogenin D were mainly included PI3K-Akt signaling pathway, while Calcium and MAPK signaling pathway also appeared in the KEGG analysis results of prosapogenin D (Figure 15), which also proved that PG saponin prosapogenin D played an anti-obesity role, validating the plausibility of the experiment. The gene discussed is AKT1; the disease is obesity due to melanocortin 4 receptor deficiency.