Logistic regression analysis (Table S7) for the association of PNPLA3 rs738409 C>G with different severities of hepatic steatosis in MASLD was assessed, S2 was positively correlated with the GG genotype (OR = 4.947, 95%CI 1.941–12.609, p = 0.001), S3 was positively correlated with the GG genotype (OR = 3.525, 95%CI 1.312–9.471, p = 0.012), S2 did not show statistical significance with the CG genotype, and S3 was positively correlated with the CG genotype (OR = 2.065, 95%CI 1.226–3.478, p = 0.006). Here, PNPLA3 is linked to fatty liver disease.