The TGF-β release was significantly higher in the supernatants of the low-RCF PRF UL and low-RCF PRF BC (~800 and ~1200 pg/mL) than in the UL and BC fractions of the PRF generated via high RCF (~170 and ~200 pg/mL) (Figure 5B). The gene discussed is TGFB1; the disease is breast cancer.