In a murine model of MASH, the combination of a high fat, sucrose, and cholesterol diet (HFSCD) and low doses of carbon tetrachloride (CCl4) increased the production of ROS and the levels of mitogen-activated kinase protein (MAPK), which in turn triggered the powerful pro-inflammatory signal mediated by NF-kB and NLRP3 activation, leading to further inflammation and fibrosis [56]. Here, NLRP3 is linked to metabolic dysfunction-associated steatohepatitis.