Another potential limit, which would have brought some information related to the more precise tissue localization of CGRP and CRLR, is related to the possibility of performing double immunohistochemical or fluorescent reactions to indicate the presence of possible colocalizations; this aspect is important to establish the tissue sources of the proteins and the autocrine and paracrine mechanisms involved in tumor stimulation and that can be carried out in future studies. The gene discussed is CALCA; the disease is neoplasm.