Transient overexpression of miR-145-5p in RWPE1, DU145 and PC3 prostate cancer cell lines (Supplementary Figure S4) consistently led to decreased MYO6 mRNA and protein levels, which was validated by a significant negative correlation between miR-145-5p and MYO6 expression in TCGA PRAD samples (Figure 2). Here, MYO6 is linked to Familial prostate cancer.